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- Keywords isolated rat islets;insulin;secretion responsiveness; 分离的大鼠胰岛;胰岛素;分泌反应性;
- Effects of clozapine and its metabolites on the intracellular calcium concentration in cells of isolated rat islets 氯氮平及其代谢产物对大鼠胰岛细胞胞内钙的影响
- isolated rat islets 胰岛细胞
- Method Rat islets were isolated with in situ ductal collagenase distention, stationary digestion and dextran gradient separation. 方法本实验经胰管注射胶原酶、胰腺静止消化分离成年大鼠胰岛,葡聚糖离心纯化,获得胰岛细胞,进行记数和功能鉴定。
- This method produced a high yield of viable purified rat islets. 异种移植可逆转实验性糖尿病小鼠的高血糖达一周。
- Methods The SD rat islets were isolated by digestive method with collagenase and DNase, and incubated in RPMI 1640 culture medium overnight. Multiscreen Assay System was used to observe the effects of CDS on the function of the isolated islets. 方法 胶原酶和DNA酶联合消化法分离SD大鼠胰岛 ,RPMI164 0组织培养液过夜培养 ,采用MilliporeMultiscreen系统观察CDS对胰岛功能的影响。
- The best methods of preparation of adult swine and rat islets were found out. 选出了制备适合猪、鼠胰岛的最佳方法。
- Objective To st,udy the effects of 2-APB on store operated calcium current (Isoc) in isolated rat hepatocytes. 目的探讨钙池操纵的钙通道(SOC)抑制剂(2APB)对大鼠肝细胞SOC电流(Isoc)的影响。 方法急性分离大鼠肝细胞,应用全细胞膜片钳记录技术测定大鼠肝细胞Isoc,并观察2APB对该电流的影响。
- Objective To choose the best method of preparation of adult swine and rat islets. 摘要目的选择最佳猪、鼠胰岛的制备方法。
- Results The best methods of preparation of adult swine and rat islets were found out. 结果选出了制备适合猪、鼠胰岛的最佳方法。
- Objective This paper is to study the mechanism of HI 6,PAM Cl,PAM I and obidoxime against the inhibition of DDVP on isolated rat phrenic diaphragm. 目的研究HI－6、PAM－Cl、PAM－I和双复磷等肟类药物对抗敌敌畏抑制离体大鼠膈肌的作用机制。
- Objective To choose the bestmethod of preparation of adult swine and rat islets. 目的选择最佳猪、鼠胰岛的制备方法。
- Noradrenaline decreased I_(K) distincily.Isoprenaline and acetylcholine showed no effect on I_(K) in isolated rat hepatocytes. 去甲肾上腺素明显降低I_(K)，异丙肾上腺素和乙酰胆碱对I_(K)无影响。
- Second, in isolated rat adipose cells, glucose transporter 4 trafficking can be visualized by transfection of HA-GLUT4-GFP. 然后,在大鼠的脂肪细胞中,通过转染HA-GLUT4-GFP来观察葡萄糖转运蛋白4的转运过程。
- Objective To explore the protective effects of WIN55212-2,an agonist of cannabinoid receptor,on ischemia-reperfusion injury in isolated rat hearts. 目的探讨大麻素受体激动剂WIN55212-2对体外大鼠心肌缺血再灌注损伤的保护作用。
- Methods The SD rats islets were isolated by digestive method with collagenase and DNase. Millipore Multiscreen Assay System was used to observe the effects of phentolamine on the function of isolated islets. 方法 胶原酶和DNA酶联合消化法分离SD大鼠胰岛 ,RP MI164 0组织培养液过夜培养 ,采用MilliporeMultiscreen系统观察不同浓度葡萄糖及酚妥拉明对胰岛功能的影响。
- Objective To study and compare the effect of shuichandpu and shichangpu on contractile of isolated rat gastric muscle strips. 目的:观察水菖蒲和石菖蒲对大鼠离体胃平滑肌条运动的影响,并比较二者作用的异同。
- Keywords isolated perfused kidney;urate excretion;rat; 离体肾脏灌流;尿酸排泄;大鼠;
- Methods Using different techniques of collagenase digestion and discontinued density gradient centrifugation, the adult swine and rat islets were prepared. 方法采用不同的胶原酶消化法及不连续密度梯度纯化法。
- METHODS: Cell membrane of isolated rat pancreatic acini were permeabilized using streptolysin O (SLO). Enzymes secretions were indicated by detecting total protein secretions. 方法:分离大鼠胰腺腺泡细胞,用链球菌溶血素-O(SLO)通透细胞膜,检测腺泡分泌蛋白量标志腺泡酶分泌功能。
